Hotspot

ndhF

SSC · 105219-107438 · 2220 bp

Back to Ampelocalamus

Pi 0.00411

MarkerSeek score 43.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 41
pi: 0.00411
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAAATCGCGGAAAACCGAAC	ACGAAACATGAGAGCTGCTT	45.0	45.0	58.0	57.8	2690-2753 bp	1.0	82.0
ndhF_p2	ACCCATCAATGCATGGTGTT	ACGAAACATGAGAGCTGCTT	45.0	45.0	58.1	57.8	2791-2854 bp	1.0	81.8
ndhF_p3	AATCGCGGAAAACCGAACTA	ACGAAACATGAGAGCTGCTT	45.0	45.0	57.9	57.8	2688-2751 bp	1.0	81.7
ndhF_p4	TCGCGGAAAACCGAACTATT	ACGAAACATGAGAGCTGCTT	45.0	45.0	57.9	57.8	2686-2749 bp	1.0	81.7
ndhF_p5	AAAATCGCGGAAAACCGAAC	CTACGGCCTATCCCCCATAT	45.0	55.0	58.0	57.8	2936-2999 bp	0.846154	74.4