Hotspot

rps15-ndhF

IRb · 104774-105218 · 445 bp

Back to Ampelocalamus

Pi 0.0227

MarkerSeek score 72.00

Primer available yes

Label rps15-ndhF

Feature Metadata

label_name: rps15-ndhF
feature_type: igs
region: IRb
length_bp: 445
variable_sites: 33
pi: 0.0227
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhF_p1	GTTTGCTGGCTTATTTGGCA	AAATATGTAGGGGGAGGGCG	45.0	55.0	57.5	58.9	584-637 bp	1.0	77.5
rps15-ndhF_p2	GTTTGCTGGCTTATTTGGCA	GTAGGGGGAGGGCGAATAT	45.0	57.9	57.5	57.9	578-631 bp	1.0	76.3
rps15-ndhF_p3	GTTTGCTGGCTTATTTGGCA	GTAGGGGGAGGGCGAATATC	45.0	60.0	57.5	59.1	578-631 bp	1.0	76.2
rps15-ndhF_p4	CGTTTGCTGGCTTATTTGGC	AAATATGTAGGGGGAGGGCG	50.0	55.0	58.7	58.9	585-638 bp	1.0	75.9
rps15-ndhF_p5	ACGTTTGCTGGCTTATTTGG	AAATATGTAGGGGGAGGGCG	45.0	55.0	57.3	58.9	586-639 bp	1.0	75.3