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psbE-petL

LSC · 64814-66040 · 1227 bp

Back to Ampelocalamus

Pi 0.00757

MarkerSeek score 62.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1227
variable_sites: 49
pi: 0.00757
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.0	58.5	1382-1402 bp	0.923077	76.3
psbE-petL_p2	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	45.0	50.0	58.0	58.5	1380-1400 bp	0.923077	76.3
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1371-1391 bp	0.923077	76.1
psbE-petL_p4	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1369-1389 bp	0.923077	76.0
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	58.2	58.5	1373-1393 bp	0.923077	74.6