Hotspot

petA-psbJ

LSC · 63061-64044 · 984 bp

Back to Ampelocalamus

Pi 0.0108

MarkerSeek score 64.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 984
variable_sites: 57
pi: 0.0108
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	TCCTCTTTGGCTGATAGGTACT	50.0	45.5	57.9	58.0	1186-1192 bp	1.0	68.0
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	ACGGTTCATATTCTGGATTGGG	50.0	45.5	57.9	58.2	1121-1127 bp	1.0	66.8
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	CGGTTCATATTCTGGATTGGGT	50.0	45.5	57.9	58.2	1120-1126 bp	1.0	66.8
petA-psbJ_p4	GGCCTTTTGTTCTTCTTCGC	TTCCTCTTTGGCTGATAGGT	50.0	45.0	57.9	55.8	1187-1193 bp	1.0	66.6
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	CCTCTTTGGCTGATAGGTACTG	50.0	50.0	57.9	57.9	1185-1191 bp	0.923077	64.3