Hotspot

ndhC-trnV-UAC

LSC · 52526-53281 · 756 bp

Back to Ampelocalamus

Pi 0.0102

MarkerSeek score 65.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 31
pi: 0.0102
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTCACTAATCGGGGCTA	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.5	57.9	889-1027 bp	1.0	79.9
ndhC-trnV-UAC_p2	TCCTTCACTAATCGGGGCTA	CGGTTCGAATCCGTATAGCC	50.0	55.0	57.5	58.3	890-1028 bp	1.0	78.1
ndhC-trnV-UAC_p3	TGGTCCTTCACTAATCGGGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.8	57.9	892-1030 bp	1.0	77.2
ndhC-trnV-UAC_p4	CTGGTCCTTCACTAATCGGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	57.4	57.9	893-898 bp	0.923077	73.3
ndhC-trnV-UAC_p5	CTGGTCCTTCACTAATCGGG	CGGTTCGAATCCGTATAGCC	55.0	55.0	57.4	58.3	894-899 bp	0.923077	71.4