Hotspot

rpoC2-rps2

LSC · 31752-32057 · 306 bp

Back to Ampelocalamus

Pi 0.0129

MarkerSeek score 63.00

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 306
variable_sites: 16
pi: 0.0129
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	ATGAGACATCAGAACCCCCA	ACTCCCGCCTCTATCATCTC	50.0	55.0	58.0	58.1	379-422 bp	1.0	86.8
rpoC2-rps2_p2	TGAGACATCAGAACCCCCAT	ACTCCCGCCTCTATCATCTC	50.0	55.0	58.0	58.1	378-421 bp	1.0	86.8
rpoC2-rps2_p3	ATGAGACATCAGAACCCCCA	TGAACTCCCGCCTCTATCAT	50.0	50.0	58.0	57.9	382-425 bp	1.0	86.7
rpoC2-rps2_p4	TGAGACATCAGAACCCCCAT	ATGAACTCCCGCCTCTATCA	50.0	50.0	58.0	57.9	382-425 bp	1.0	86.7
rpoC2-rps2_p5	TGAGACATCAGAACCCCCAT	TGAACTCCCGCCTCTATCAT	50.0	50.0	58.0	57.9	381-424 bp	1.0	86.7