Hotspot

trnfM-CAU-trnT-GGU

LSC · 13536-16313 · 2778 bp

Back to Ampelocalamus

Pi 0.0107

MarkerSeek score 70.20

Primer available yes

Label trnfM-CAU-trnT-GGU

Feature Metadata

label_name: trnfM-CAU-trnT-GGU
feature_type: igs
region: LSC
length_bp: 2778
variable_sites: 152
pi: 0.0107
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-trnT-GGU_p1	CCTCGTGAGCTACCAAACTG	CCATGGCATTACTCTACCACC	55.0	52.4	58.3	58.1	2911-2987 bp	1.0	74.2
trnfM-CAU-trnT-GGU_p2	CCTCGTGAGCTACCAAACTG	GACTTACGTCTTACCATGGCA	55.0	47.6	58.3	57.8	2924-3000 bp	1.0	73.5
trnfM-CAU-trnT-GGU_p3	CCTCAAGGTTATGAGCCTCG	CCATGGCATTACTCTACCACC	55.0	52.4	57.8	58.1	2926-2972 bp	0.923077	71.5
trnfM-CAU-trnT-GGU_p4	CCTCAAGGTTATGAGCCTCG	GACTTACGTCTTACCATGGCA	55.0	47.6	57.8	57.8	2939-2985 bp	0.923077	70.7
trnfM-CAU-trnT-GGU_p5	CCTCAAGGTTATGAGCCTCG	TGACTTACGTCTTACCATGGC	55.0	47.6	57.8	57.8	2940-2986 bp	0.923077	70.7