Hotspot

rps16

LSC · 5513-7224 · 1712 bp

Back to Amorphophallus

Pi 0.0755

MarkerSeek score 80.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1712
variable_sites: 423
pi: 0.0755
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TATGGGTGGGGATAAACGGA	ATCAATTCCATCCCGGTTCG	50.0	50.0	57.8	58.0	2018-2395 bp	0.5	70.2
rps16_p2	TATGGGTGGGGATAAACGGA	GTTAATGTAGCGACTCGGGG	50.0	55.0	57.8	58.2	2624-2984 bp	0.454545	67.0
rps16_p3	AAAGCAAGAAAAGGTGCGTG	ATCAATTCCATCCCGGTTCG	45.0	50.0	57.8	58.0	1755-2132 bp	0.227273	53.5
rps16_p4	CAAAGCAAGAAAAGGTGCGT	ATCAATTCCATCCCGGTTCG	45.0	50.0	57.8	58.0	1756-2133 bp	0.227273	53.5
rps16_p5	AAAGCAAGAAAAGGTGCGTG	GTTAATGTAGCGACTCGGGG	45.0	55.0	57.8	58.2	2371-2753 bp	0.227273	52.1