Hotspot

rpl16

LSC · 89229-91407 · 2179 bp

Back to Amorphophallus

Pi 0.0806

MarkerSeek score 79.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 2179
variable_sites: 656
pi: 0.0806
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CCGTTTTCACGCTTGAGTTC	CGACGGAAAAGAAGTTGCAC	50.0	50.0	58.0	58.0	1946-2989 bp	0.409091	68.1
rpl16_p2	CCGTTTTCACGCTTGAGTTC	GACGGAAAAGAAGTTGCACG	50.0	50.0	58.0	58.0	1945-2988 bp	0.409091	68.1
rpl16_p3	AATTTTCGAGCCCCGCTATT	GACGGAAAAGAAGTTGCACG	45.0	50.0	57.9	58.0	1780-2862 bp	0.363636	65.2
rpl16_p4	TCTCAATTCCCTGGCGATTG	CGACGGAAAAGAAGTTGCAC	50.0	50.0	58.0	58.0	2046-2946 bp	0.272727	58.6
rpl16_p5	TCTCAATTCCCTGGCGATTG	GACGGAAAAGAAGTTGCACG	50.0	50.0	58.0	58.0	2045-2945 bp	0.272727	58.6