Hotspot

ndhB

IRa · 161909-164118 · 2210 bp

Back to Amorphophallus

Pi 0.0351

MarkerSeek score 79.60

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: IRa
length_bp: 2210
variable_sites: 833
pi: 0.0351
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	ATTGGGACTTTTTCGGCGAT	TAAGTTCATAACGCCAGCCC	45.0	50.0	58.2	58.0	2351-2378 bp	1.0	90.4
ndhB_p2	CAATTGGGACTTTTTCGGCG	TAAGTTCATAACGCCAGCCC	50.0	50.0	58.3	58.0	2353-2380 bp	1.0	89.3
ndhB_p3	GGCGATTGGATGCAGTTACT	TAAGTTCATAACGCCAGCCC	50.0	50.0	58.3	58.0	2337-2364 bp	1.0	89.1
ndhB_p4	AATTGGGACTTTTTCGGCGA	TAAGTTCATAACGCCAGCCC	45.0	50.0	58.4	58.0	2352-2379 bp	1.0	88.6
ndhB_p5	TTGGGACTTTTTCGGCGATT	TAAGTTCATAACGCCAGCCC	45.0	50.0	58.4	58.0	2350-2377 bp	1.0	88.6