Hotspot

ycf1-ndhF

IRb · 125068-126802 · 1735 bp

Back to Amorphophallus

Pi 0.284

MarkerSeek score 69.00

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: IRb
length_bp: 1735
variable_sites: 1143
pi: 0.284
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.40
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	TCGAAGAAGAGAGATGCGTC	TATTTAGTAGGGGGAGGGCG	50.0	55.0	57.2	58.0	376-2863 bp	0.590909	68.8
ycf1-ndhF_p2	CGAAGAAGAGAGATGCGTCT	TATTTAGTAGGGGGAGGGCG	50.0	55.0	57.2	58.0	375-2862 bp	0.590909	68.7
ycf1-ndhF_p3	TCGAAGAAGAGAGATGCGTC	TGGTTGGCTCCATCGAAAAA	50.0	45.0	57.2	58.0	775-2879 bp	0.545455	67.0
ycf1-ndhF_p4	CGAAGAAGAGAGATGCGTCT	TGGTTGGCTCCATCGAAAAA	50.0	45.0	57.2	58.0	774-2878 bp	0.545455	67.0
ycf1-ndhF_p5	TCGAAGAAGAGAGATGCGTC	TGCTTTTGGTCAAGGGGAAA	50.0	45.0	57.2	57.8	818-2922 bp	0.545455	65.8