Hotspot

rpoB-trnC-GCA

LSC · 29731-31263 · 1533 bp

Back to Amorphophallus

Pi 0.0361

MarkerSeek score 81.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1533
variable_sites: 240
pi: 0.0361
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCTCATTTTCATCCCGGAG	GCGACACCCAGATTTGAACT	55.0	50.0	58.0	58.5	1555-1987 bp	0.954545	86.2
rpoB-trnC-GCA_p2	CTCATTTTCATCCCGGAGCA	GCGACACCCAGATTTGAACT	50.0	50.0	58.0	58.5	1553-1985 bp	0.954545	86.0
rpoB-trnC-GCA_p3	CCCTCATTTTCATCCCGGAG	CCTTACCACTTGGCCATGTC	55.0	55.0	58.0	58.5	1509-1941 bp	0.954545	85.7
rpoB-trnC-GCA_p4	CCCTCATTTTCATCCCGGAG	TAAAGGATTTGCAGTCCCCC	55.0	50.0	58.0	57.5	1530-1840 bp	0.909091	83.9
rpoB-trnC-GCA_p5	CTCATTTTCATCCCGGAGCA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	57.5	1528-1838 bp	0.909091	83.8