Hotspot

trnS-GCU-trnG-UCC

LSC · 9621-11528 · 1908 bp

Back to Amorphophallus

Pi 0.0991

MarkerSeek score 85.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1908
variable_sites: 667
pi: 0.0991
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GAGAAAGCGGAGAAAGAGGG	AGGACGAATCACACTTTTACCA	55.0	40.9	58.0	57.7	1040-2954 bp	0.954545	71.3
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AGGACGAATCACACTTTTACCA	45.0	40.9	58.0	57.7	985-2899 bp	0.954545	71.1
trnS-GCU-trnG-UCC_p3	GAGAAAGCGGAGAAAGAGGG	GGACGAATCACACTTTTACCAC	55.0	45.5	58.0	57.6	1039-2953 bp	0.954545	70.8
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	GGACGAATCACACTTTTACCAC	45.0	45.5	58.0	57.6	984-2898 bp	0.954545	70.7
trnS-GCU-trnG-UCC_p5	TCAAGTGACCAGAGAAAGCG	AGGACGAATCACACTTTTACCA	50.0	40.9	57.8	57.7	1051-2965 bp	0.909091	68.4