Hotspot

ndhF

SSC · 113912-116155 · 2244 bp

Back to Amorpha

Pi 0.00416

MarkerSeek score 59.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 14
pi: 0.00416
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAACCCCCTCATTTTGCCAA	ACAAAAGAGATAAAACCGGCG	45.0	42.9	58.2	57.2	2356-2357 bp	1.0	64.4
ndhF_p2	AAACCCCCTCATTTTGCCAA	CAAAAGAGATAAAACCGGCGA	45.0	42.9	58.2	56.9	2355-2356 bp	1.0	62.3
ndhF_p3	AACCCCCTCATTTTGCCAAG	ACAAAAGAGATAAAACCGGCG	50.0	42.9	58.6	57.2	2355-2356 bp	1.0	60.7
ndhF_p4	CCCCCTCATTTTGCCAAGTT	ACAAAAGAGATAAAACCGGCG	50.0	42.9	58.6	57.2	2353-2354 bp	1.0	60.7
ndhF_p5	TAAACCCCCTCATTTTGCCA	ACAAAAGAGATAAAACCGGCG	45.0	42.9	57.3	57.2	2357-2358 bp	1.0	60.3