Hotspot

ndhA

SSC · 123296-125619 · 2324 bp

Back to Amorpha

Pi 0.00287

MarkerSeek score 45.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2324
variable_sites: 9
pi: 0.00287
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CTCGGACTGTTTGTTGACCA	AAGAGTTGAAGCCCCAAAGG	50.0	50.0	58.1	58.0	2632-2650 bp	1.0	80.4
ndhA_p2	CTCGGACTGTTTGTTGACCA	CCCTGGAGATGGAAAATCCG	50.0	55.0	58.1	58.0	2571-2589 bp	1.0	80.2
ndhA_p3	CGGCCCAAAGCAATTTGATT	CCCTGGAGATGGAAAATCCG	45.0	55.0	57.9	58.0	2950-2968 bp	1.0	79.8
ndhA_p4	CTCGGACTGTTTGTTGACCA	TCCGACTTTTGAATTGCCGA	50.0	45.0	58.1	58.1	2668-2686 bp	1.0	79.5
ndhA_p5	ACCAATGTACCTTGCAGCTC	AAGAGTTGAAGCCCCAAAGG	50.0	50.0	58.2	58.0	2649-2667 bp	1.0	79.4