Hotspot

rpl32-trnL-UAG

SSC · 117010-117722 · 713 bp

Back to Amorpha

Pi 0.00562

MarkerSeek score 62.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 713
variable_sites: 6
pi: 0.00562
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAAGGGGTATTGGACAGCA	GATAAGATGCTATGCCGCCA	50.0	50.0	58.3	57.9	889-895 bp	1.0	77.3
rpl32-trnL-UAG_p2	CGCAATCCATTTCTACGGGT	GATAAGATGCTATGCCGCCA	50.0	50.0	58.3	57.9	850-856 bp	1.0	77.3
rpl32-trnL-UAG_p3	AGAAGGGGTATTGGACAGCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.3	57.9	835-841 bp	1.0	77.1
rpl32-trnL-UAG_p4	CGCAATCCATTTCTACGGGT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.3	57.9	796-802 bp	1.0	77.1
rpl32-trnL-UAG_p5	AGAAGGGGTATTGGACAGCA	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.3	57.8	828-834 bp	1.0	76.6