Hotspot

rpl36-rps8

LSC · 83313-83752 · 440 bp

Back to Amorpha

Pi 0.00455

MarkerSeek score 50.50

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 440
variable_sites: 3
pi: 0.00455
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	ACTATAATTCGCCCCCGTCT	GAGAATCGGCGGAGAAGTTT	50.0	50.0	58.3	58.0	550-552 bp	1.0	78.2
rpl36-rps8_p2	ACTATAATTCGCCCCCGTCT	TAGAGAGAATCGGCGGAGAA	50.0	50.0	58.3	57.7	554-556 bp	1.0	75.5
rpl36-rps8_p3	ACTATAATTCGCCCCCGTCT	TTAGAGAGAATCGGCGGAGA	50.0	50.0	58.3	57.7	555-557 bp	1.0	75.5
rpl36-rps8_p4	ACTATAATTCGCCCCCGTCT	AGAATCGGCGGAGAAGTTTT	50.0	45.0	58.3	57.5	549-551 bp	1.0	74.5
rpl36-rps8_p5	CGTCTACGGATCAATCGACA	GAGAATCGGCGGAGAAGTTT	50.0	50.0	57.2	58.0	535-537 bp	1.0	74.5