Hotspot

trnE-UUC-trnT-GGU

LSC · 77291-78125 · 835 bp

Back to Amorpha

Pi 0.006

MarkerSeek score 62.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 7
pi: 0.006
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGAGATGTCCTGGACCAC	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	922-936 bp	1.0	79.6
trnE-UUC-trnT-GGU_p2	GAGAGATGTCCTGGACCACT	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	921-935 bp	1.0	79.6
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	974-988 bp	1.0	79.4
trnE-UUC-trnT-GGU_p4	AGAGAGATGTCCTGGACCAC	CCATGGCGTTACTCTACCAC	55.0	55.0	58.1	58.1	901-915 bp	1.0	79.2
trnE-UUC-trnT-GGU_p5	GAGAGATGTCCTGGACCACT	CCATGGCGTTACTCTACCAC	55.0	55.0	58.1	58.1	900-914 bp	1.0	79.2