Hotspot

trnD-GUC-trnY-GUA

LSC · 76622-77073 · 452 bp

Back to Amorpha

Pi 0.0119

MarkerSeek score 51.10

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 452
variable_sites: 8
pi: 0.0119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	CTGGTTCAAATCCAGCTCGA	50.0	50.0	58.5	57.9	504-506 bp	1.0	77.0
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	ATGGATTCTTCCTGGGTCGA	50.0	50.0	58.5	58.1	577-579 bp	1.0	76.6
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	TGGATTCTTCCTGGGTCGAT	50.0	50.0	58.5	58.1	576-578 bp	1.0	76.6
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	TGGTTCAAATCCAGCTCGAC	50.0	50.0	58.5	58.2	503-505 bp	1.0	76.2
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	520-522 bp	1.0	75.8