Hotspot

trnC-GCA-petN

LSC · 73885-74703 · 819 bp

Back to Amorpha

Pi 0.0049

MarkerSeek score 56.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 819
variable_sites: 5
pi: 0.0049
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	57.9	58.0	926-938 bp	1.0	81.0
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	870-882 bp	1.0	80.8
trnC-GCA-petN_p3	TTTTGTATCGTTTTGGCGGC	CAGCCCAAGCAAGACTTACT	45.0	50.0	57.9	57.8	920-932 bp	1.0	79.5
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	864-876 bp	1.0	79.4
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	888-900 bp	1.0	78.6