Hotspot

trnF-GAA-ndhJ

LSC · 50145-50914 · 770 bp

Back to Amorpha

Pi 0.00433

MarkerSeek score 61.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 770
variable_sites: 5
pi: 0.00433
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TTGGATAGGATGGCCTTTGC	55.0	50.0	58.0	57.9	909-930 bp	1.0	79.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTGC	50.0	50.0	58.1	57.9	873-894 bp	1.0	79.6
trnF-GAA-ndhJ_p3	AGAATGGTCGGGATAGCTCA	TTGGATAGGATGGCCTTTGC	50.0	50.0	57.9	57.9	918-939 bp	1.0	79.2
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	TTGGATAGGATGGCCTTTGC	55.0	50.0	57.9	57.9	897-918 bp	1.0	78.9
trnF-GAA-ndhJ_p5	TAGGTCAAGAATGGTCGGGA	TTGGATAGGATGGCCTTTGC	50.0	50.0	57.8	57.9	925-946 bp	1.0	78.0