Hotspot

rps11-trnG-GCC

LSC · 37270-38157 · 888 bp

Back to Amorpha

Pi 0.00601
MarkerSeek score 54.80
Primer available yes
Label rps11-trnG-GCC

Feature Metadata

label_name
rps11-trnG-GCC
feature_type
igs
region
LSC
length_bp
888
variable_sites
8
pi
0.00601
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-trnG-GCC_p1 CGTCCATTTTTACGCGAACC CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.1 57.8 979-1009 bp 1.0 79.2
rps11-trnG-GCC_p2 ACGTCCATTTTTACGCGAAC CGTCTTCTCCTTGGCAAAGA 45.0 50.0 57.4 57.8 980-1010 bp 1.0 75.5
rps11-trnG-GCC_p3 GTCCATTTTTACGCGAACCA CGTCTTCTCCTTGGCAAAGA 45.0 50.0 57.0 57.8 978-1008 bp 1.0 72.4
rps11-trnG-GCC_p4 CGAGAATGCTTACGTGAACC CGTCTTCTCCTTGGCAAAGA 50.0 50.0 56.9 57.8 1003-1033 bp 1.0 71.6
rps11-trnG-GCC_p5 CGTCCATTTTTACGCGAACC CGGATAGCGGGAATCGAAC 50.0 57.9 58.1 58.2 1001-1031 bp 1.0 71.0