Hotspot

petA-psbJ

LSC · 19727-20894 · 1168 bp

Back to Amorpha

Pi 0.00343

MarkerSeek score 61.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1168
variable_sites: 6
pi: 0.00343
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	CATATTCCGGATTGGGCTCA	50.0	50.0	57.8	57.4	1306-1308 bp	1.0	74.0
petA-psbJ_p2	CGTTACGTGTCCAAGGTCTC	CATATTCCGGATTGGGCTCA	55.0	50.0	58.3	57.4	1311-1313 bp	1.0	73.5
petA-psbJ_p3	GATCCGTTACGTGTCCAAGG	CATATTCCGGATTGGGCTCA	55.0	50.0	58.4	57.4	1315-1317 bp	1.0	73.0
petA-psbJ_p4	CGTGTCCAAGGTCTCTTGTT	TATTCCGGATTGGGCTCATC	50.0	50.0	57.8	57.1	1304-1306 bp	1.0	71.7
petA-psbJ_p5	CGTGTCCAAGGTCTCTTGTT	TCATATTCCGGATTGGGCTC	50.0	50.0	57.8	57.1	1307-1309 bp	1.0	71.7