Hotspot

psbK-trnQ-UUG

LSC · 11247-11994 · 748 bp

Back to Amorpha

Pi 0.00182

MarkerSeek score 61.70

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 2
pi: 0.00182
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	GAATAAAGGGCGGGGTGAAT	GGATATCCTCTGGGACGGAA	50.0	55.0	57.9	58.0	869-882 bp	1.0	80.9
psbK-trnQ-UUG_p2	GAATAAAGGGCGGGGTGAAT	ATATCCTCTGGGACGGAAGG	50.0	55.0	57.9	58.0	867-880 bp	1.0	80.9
psbK-trnQ-UUG_p3	GAATAAAGGGCGGGGTGAAT	ATGGATATCCTCTGGGACGG	50.0	55.0	57.9	58.1	871-884 bp	1.0	80.6
psbK-trnQ-UUG_p4	GGGCGGGGTGAATACAAATA	GGATATCCTCTGGGACGGAA	50.0	55.0	57.6	58.0	862-875 bp	1.0	78.7
psbK-trnQ-UUG_p5	GGGCGGGGTGAATACAAATA	ATATCCTCTGGGACGGAAGG	50.0	55.0	57.6	58.0	860-873 bp	1.0	78.6