Hotspot

trnK-UUU-rbcL

LSC · 4363-5399 · 1037 bp

Back to Amorpha

Pi 0.00514

MarkerSeek score 62.00

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 1037
variable_sites: 8
pi: 0.00514
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	AGCCGAGTACTCTACCATTGA	AGTCCTGAATTAGCTGCTGC	47.6	50.0	58.0	58.0	1144-1164 bp	1.0	72.2
trnK-UUU-rbcL_p2	AGCCGAGTACTCTACCATTGA	GAGGCTAGCAAATGGAGTCC	47.6	55.0	58.0	58.0	1159-1179 bp	1.0	72.1
trnK-UUU-rbcL_p3	AATGGAGTCGCGCTTAAAAG	AGTCCTGAATTAGCTGCTGC	45.0	50.0	56.8	58.0	1162-1182 bp	1.0	70.8
trnK-UUU-rbcL_p4	AATGGAGTCGCGCTTAAAAG	GAGGCTAGCAAATGGAGTCC	45.0	55.0	56.8	58.0	1177-1197 bp	1.0	70.8
trnK-UUU-rbcL_p5	AGCCGAGTACTCTACCATTGA	GGCTAGCAAATGGAGTCCTG	47.6	55.0	58.0	58.3	1157-1177 bp	1.0	69.8