Hotspot

rps19

IRa · 163350-163628 · 279 bp

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Pi 0.0108
MarkerSeek score 36.30
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
IRa
length_bp
279
variable_sites
6
pi
0.0108
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 GGGTAGAGCCGGATCTAAGT TGATTTTGATGATGATGTAATAGA 55.0 25.0 58.0 52.1 907 bp 0.25 10.0
rps19_p2 TGGGTAGAGCCGGATCTAAG TGATTTTGATGATGATGTAATAGA 55.0 25.0 58.0 52.1 908 bp 0.25 10.0
rps19_p3 GTTATCCTGCGCTTGGAAGA TGATTTTGATGATGATGTAATAGA 50.0 25.0 58.0 52.1 770 bp 0.25 10.0
rps19_p4 GAGCCGGATCTAAGTGTTGG TGATTTTGATGATGATGTAATAGA 55.0 25.0 58.1 52.1 902 bp 0.25 10.0
rps19_p5 AAAAGTTTGGGTAGAGCCGG TGATTTTGATGATGATGTAATAGA 50.0 25.0 58.1 52.1 915 bp 0.25 10.0