Hotspot

psaC-ndhE

SSC · 125715-126429 · 715 bp

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Pi 0.0326
MarkerSeek score 71.90
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
715
variable_sites
36
pi
0.0326
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.92
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 CGTCCCACGGTATCATTTCC TCAACACGTATCAACCAATCGA 55.0 40.9 58.4 58.1 788-851 bp 1.0 67.5
psaC-ndhE_p2 GGCAAGCTCGTACACATTGA TCAACACGTATCAACCAATCGA 50.0 40.9 58.6 58.1 755-818 bp 1.0 66.5
psaC-ndhE_p3 CGTCCCACGGTATCATTTCC CAACACGTATCAACCAATCGA 55.0 42.9 58.4 56.7 787-850 bp 1.0 65.4
psaC-ndhE_p4 CGTCCCACGGTATCATTTCC TCAACACGTATCAACCAATCG 55.0 42.9 58.4 56.7 788-851 bp 1.0 65.4
psaC-ndhE_p5 GTCCCACGGTATCATTTCCA TCAACACGTATCAACCAATCGA 50.0 40.9 57.3 58.1 787-850 bp 1.0 65.2