Hotspot

trnL-UAG-ccsA

SSC · 122453-122560 · 108 bp

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Pi 0.0324
MarkerSeek score 71.20
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
108
variable_sites
7
pi
0.0324
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA TCGAAAAAGAGATATGAGTCA 50.0 33.3 57.9 52.1 204-218 bp 1.0 46.6
trnL-UAG-ccsA_p2 GGTAGACACGCTGCTCTTAG TCGAAAAAGAGATATGAGTCA 55.0 33.3 57.8 52.1 206-220 bp 1.0 46.6
trnL-UAG-ccsA_p3 GTAGACACGCTGCTCTTAGG TCGAAAAAGAGATATGAGTCA 55.0 33.3 57.8 52.1 205-219 bp 1.0 46.6
trnL-UAG-ccsA_p4 AAATTGGTAGACACGCTGCT TCGAAAAAGAGATATGAGTCA 45.0 33.3 57.8 52.1 211-225 bp 1.0 46.5
trnL-UAG-ccsA_p5 TAAGCCGCCATGGTGAAATT TCGAAAAAGAGATATGAGTCA 45.0 33.3 58.2 52.1 226-240 bp 1.0 46.4