Hotspot

psbE-petL

LSC · 68764-70015 · 1252 bp

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Pi 0.0216
MarkerSeek score 73.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1252
variable_sites
48
pi
0.0216
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AAGCAGCCAGTAGAAAACCG 50.0 50.0 57.9 58.5 1356-1432 bp 1.0 82.3
psbE-petL_p2 CAACCCGCAATGAATAGGGA GCAGCCAGTAGAAAACCGAA 50.0 50.0 57.9 58.5 1354-1430 bp 1.0 82.3
psbE-petL_p3 ACTGACGAATAACCAACCCG AAGCAGCCAGTAGAAAACCG 50.0 50.0 57.9 58.5 1369-1445 bp 1.0 81.9
psbE-petL_p4 ACTGACGAATAACCAACCCG GCAGCCAGTAGAAAACCGAA 50.0 50.0 57.9 58.5 1367-1443 bp 1.0 81.9
psbE-petL_p5 ACGAATAACCAACCCGCAAT AAGCAGCCAGTAGAAAACCG 45.0 50.0 58.2 58.5 1365-1441 bp 1.0 81.3