Hotspot

ndhC-trnV-UAC

LSC · 53953-55534 · 1582 bp

Back to Amomum

Pi 0.0139
MarkerSeek score 74.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1582
variable_sites
43
pi
0.0139
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCTAACCGGGGCTAAAACTC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1705-1846 bp 1.0 84.1
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAACTCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1704-1845 bp 1.0 84.1
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1698-1839 bp 1.0 83.3
ndhC-trnV-UAC_p4 TTTCTCCGGTCCTTCCCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1720-1861 bp 1.0 81.7
ndhC-trnV-UAC_p5 CTAACCGGGGCTAAAACTCC GAAGGTCTACGGTTCGAGTC 55.0 55.0 58.0 57.5 1703-1844 bp 1.0 81.3