Hotspot

trnE-UUC-trnT-GGU

LSC · 33649-34543 · 895 bp

Back to Amomum

Pi 0.0338
MarkerSeek score 72.80
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
895
variable_sites
45
pi
0.0338
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 657-998 bp 1.0 85.8
trnE-UUC-trnT-GGU_p2 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 636-977 bp 1.0 85.8
trnE-UUC-trnT-GGU_p3 GCTGCCTCCTTGAAAGAGAG CCATGGCGTTACTCTACCAC 55.0 55.0 58.3 58.1 636-977 bp 1.0 85.5
trnE-UUC-trnT-GGU_p4 TGTCCTGAACCACTAGACGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.7 58.1 615-956 bp 1.0 85.4
trnE-UUC-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG TGGCGTTACTCTACCACTGA 55.0 50.0 58.3 58.1 633-974 bp 1.0 85.3