Hotspot

atpF-atpH

LSC · 13657-14180 · 524 bp

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Pi 0.0347
MarkerSeek score 74.40
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
524
variable_sites
32
pi
0.0347
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 AGTGGCCCAAGTAAACGAAA TGGTTGTGGCATTAGCACTT 45.0 45.0 57.6 58.0 592-608 bp 1.0 84.8
atpF-atpH_p2 CAGTGGCCCAAGTAAACGAA TGGTTGTGGCATTAGCACTT 50.0 45.0 58.4 58.0 593-609 bp 1.0 84.4
atpF-atpH_p3 TGGCCCAAGTAAACGAAAGA TGGTTGTGGCATTAGCACTT 45.0 45.0 57.4 58.0 590-606 bp 1.0 82.5
atpF-atpH_p4 GTGGCCCAAGTAAACGAAAG TGGTTGTGGCATTAGCACTT 50.0 45.0 57.3 58.0 591-607 bp 1.0 82.0
atpF-atpH_p5 TTAAACCCGAAACTTCCGGC TGGTTGTGGCATTAGCACTT 50.0 45.0 58.8 58.0 620-636 bp 1.0 81.1