Hotspot

clpP

LSC · 69051-71101 · 2051 bp

Back to Ammopiptanthus

Pi 0.00391

MarkerSeek score 60.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2051
variable_sites: 8
pi: 0.00391
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CCTTCCTTGCCGTTCGTTAA	ACGTTTCCACATCAAAGTGAGA	50.0	40.9	58.5	58.2	2124-2126 bp	1.0	59.0
clpP_p2	CCTTCCTTGCCGTTCGTTAA	ACGTTTCCACATCAAAGTGAG	50.0	42.9	58.5	56.8	2124-2126 bp	1.0	58.6
clpP_p3	TCCTTGCCGTTCGTTAATCT	ACGTTTCCACATCAAAGTGAGA	45.0	40.9	57.2	58.2	2121-2123 bp	1.0	56.8
clpP_p4	CCTTCCTTGCCGTTCGTTAA	ACGTTTCCACATCAAAGTGA	50.0	40.0	58.5	55.5	2124-2126 bp	1.0	56.7
clpP_p5	TCCTTGCCGTTCGTTAATCT	ACGTTTCCACATCAAAGTGAG	45.0	42.9	57.2	56.8	2121-2123 bp	1.0	56.5