Hotspot

rpl36-rps8

LSC · 79068-79550 · 483 bp

Back to Ammopiptanthus

Pi 0.0124

MarkerSeek score 63.50

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 483
variable_sites: 6
pi: 0.0124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	CCCTCCTACGGATCAATCGA	AATGACAGATCGAGAGGCTC	55.0	50.0	58.4	56.8	608-648 bp	1.0	68.3
rpl36-rps8_p2	ACTATAATTCGCCCCCTCCT	AATGACAGATCGAGAGGCTC	50.0	50.0	57.6	56.8	621-661 bp	1.0	68.0
rpl36-rps8_p3	CCTCCTACGGATCAATCGAC	AATGACAGATCGAGAGGCTC	55.0	50.0	57.3	56.8	607-647 bp	1.0	65.7
rpl36-rps8_p4	TTTTACGAACAGAGGCCCTT	AATGACAGATCGAGAGGCTC	45.0	50.0	57.1	56.8	575-615 bp	1.0	63.9
rpl36-rps8_p5	CCCTCCTACGGATCAATCGA	ACAAAGAATCGGTGGAGAAGT	55.0	42.9	58.4	57.2	584-624 bp	1.0	63.6