Hotspot

psbH-petB

LSC · 73942-75085 · 1144 bp

Back to Ammopiptanthus

Pi 0.00262

MarkerSeek score 60.30

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 1144
variable_sites: 3
pi: 0.00262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	TAATGGGTGTTGCAATGGCT	TCAGTAACGGTCGGACGATA	45.0	50.0	58.1	58.0	1274 bp	1.0	79.5
psbH-petB_p2	TAATGGGTGTTGCAATGGCT	ATACTTGCCGACCATCGATG	45.0	50.0	58.1	57.8	1358 bp	1.0	78.4
psbH-petB_p3	TAATGGGTGTTGCAATGGCT	ATCATACTTGCCGACCATCG	45.0	50.0	58.1	57.8	1361 bp	1.0	78.4
psbH-petB_p4	TAATGGGTGTTGCAATGGCT	CATACTTGCCGACCATCGAT	45.0	50.0	58.1	57.8	1359 bp	1.0	78.4
psbH-petB_p5	GGGTGTTGCAATGGCTCTAT	TCAGTAACGGTCGGACGATA	50.0	50.0	58.2	58.0	1270 bp	1.0	78.1