Hotspot

psbB-psbT

LSC · 73112-73310 · 199 bp

Back to Ammopiptanthus

Pi 0.0151

MarkerSeek score 57.00

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 3
pi: 0.0151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TTTTTGCTGGTATCGACCCA	TGGAACTTTAGGGGGTTCTCT	45.0	47.6	57.4	58.0	384 bp	1.0	68.1
psbB-psbT_p2	GTTTTTGCTGGTATCGACCC	TGGAACTTTAGGGGGTTCTCT	50.0	47.6	57.1	58.0	385 bp	1.0	65.3
psbB-psbT_p3	TGGTATCGACCCAGATTTGG	TGGAACTTTAGGGGGTTCTCT	50.0	47.6	57.0	58.0	377 bp	1.0	64.6
psbB-psbT_p4	TTTTTGCTGGTATCGACCCA	AGTTGGAACTTTAGGGGGTTC	45.0	47.6	57.4	57.5	387 bp	1.0	63.9
psbB-psbT_p5	TTTTTGCTGGTATCGACCCA	GTTGGAACTTTAGGGGGTTCT	45.0	47.6	57.4	57.5	386 bp	1.0	63.9