Hotspot

psaA-ycf3

LSC · 50465-51193 · 729 bp

Back to Ammopiptanthus

Pi 0.00412

MarkerSeek score 61.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 729
variable_sites: 3
pi: 0.00412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGCCCATTCCTCGAAAGAAG	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	877 bp	1.0	79.3
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	876 bp	1.0	79.3
psaA-ycf3_p3	TGCCCATTCCTCGAAAGAAG	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	883 bp	1.0	79.1
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	882 bp	1.0	79.1
psaA-ycf3_p5	TGCCCATTCCTCGAAAGAAG	TTGGTTGAAGATCACAGGGC	50.0	50.0	57.9	58.1	841 bp	1.0	78.7