Hotspot

atpH-atpF

LSC · 27997-28077 · 81 bp

Back to Ammopiptanthus

Pi 0.0125

MarkerSeek score 60.90

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 81
variable_sites: 1
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	TGGGCTGGTTGTAGCATTAG	CCAGTAACCCAAGCAAAGGA	50.0	50.0	57.6	57.7	171-172 bp	1.0	75.3
atpH-atpF_p2	AGGCAGAGGGAAAAATACGG	CCAGTAACCCAAGCAAAGGA	50.0	50.0	57.6	57.7	238-239 bp	1.0	75.0
atpH-atpF_p3	TGGGCTGGTTGTAGCATTAG	CCGAGCACCACACTTAGATT	50.0	50.0	57.6	57.6	245-246 bp	1.0	74.2
atpH-atpF_p4	CCTGAGGCAGAGGGAAAAAT	CCAGTAACCCAAGCAAAGGA	50.0	50.0	57.5	57.7	242-243 bp	1.0	74.2
atpH-atpF_p5	AGGCAGAGGGAAAAATACGG	CCGAGCACCACACTTAGATT	50.0	50.0	57.6	57.6	312-313 bp	1.0	74.1