Hotspot

rpoC2-rps2

LSC · 25014-25089 · 76 bp

Back to Ammopiptanthus

Pi 0.0133

MarkerSeek score 61.40

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 76
variable_sites: 1
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	TAGGAGCGGATTCATCCCTT	GTGCCATTCTCGGATTCCAT	50.0	50.0	57.9	58.0	195-196 bp	1.0	79.8
rpoC2-rps2_p2	TAGGAGCGGATTCATCCCTT	AAGGTGCCATTCTCGGATTC	50.0	50.0	57.9	58.0	198-199 bp	1.0	79.6
rpoC2-rps2_p3	TAGGAGCGGATTCATCCCTT	CCGAGTACCATGGCCAAAAT	50.0	50.0	57.9	58.2	173-174 bp	1.0	78.2
rpoC2-rps2_p4	CTAGGAGCGGATTCATCCCT	GTGCCATTCTCGGATTCCAT	55.0	50.0	58.4	58.0	196-197 bp	1.0	77.7
rpoC2-rps2_p5	TCTAGGAGCGGATTCATCCC	GTGCCATTCTCGGATTCCAT	55.0	50.0	58.4	58.0	197-198 bp	1.0	77.7