Hotspot

ndhJ-trnC-GCA

LSC · 12491-13746 · 1256 bp

Back to Ammopiptanthus

Pi 0.00558

MarkerSeek score 53.10

Primer available yes

Label ndhJ-trnC-GCA

Feature Metadata

label_name: ndhJ-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1256
variable_sites: 7
pi: 0.00558
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnC-GCA_p1	TTGGATAGGATGGCCTTTGC	ACTGCAAATCCCTTTTCCCC	50.0	50.0	57.9	58.4	1371-1402 bp	1.0	77.1
ndhJ-trnC-GCA_p2	TTGGATAGGATGGCCTTTGC	GCAAATCCCTTTTCCCCAGT	50.0	50.0	57.9	58.4	1368-1399 bp	1.0	77.1
ndhJ-trnC-GCA_p3	TTGGATAGGATGGCCTTTGC	CTGCAAATCCCTTTTCCCCA	50.0	50.0	57.9	58.4	1370-1401 bp	1.0	77.1
ndhJ-trnC-GCA_p4	TTGGATAGGATGGCCTTTGC	TGCAAATCCCTTTTCCCCAG	50.0	50.0	57.9	58.4	1369-1400 bp	1.0	77.1
ndhJ-trnC-GCA_p5	TTGGATAGGATGGCCTTTGC	AGTTCAAATCTGGGTGTCGC	50.0	50.0	57.9	58.5	1351-1382 bp	1.0	76.1