Hotspot

trnK-UUU-rbcL

LSC · 4335-5248 · 914 bp

Back to Ammopiptanthus

Pi 0.0142

MarkerSeek score 65.60

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 914
variable_sites: 13
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	AGCCGAGTACTCTACCATTGA	GCTTGCGAAGTATGGAAGGA	47.6	50.0	58.0	58.0	1003-1004 bp	1.0	72.7
trnK-UUU-rbcL_p2	AGCCGAGTACTCTACCATTGA	AGTCCTGAATTAGCTGCTGC	47.6	50.0	58.0	58.0	1021-1022 bp	1.0	72.6
trnK-UUU-rbcL_p3	AGCCGAGTACTCTACCATTGA	GAGGCTAGCAAATGGAGTCC	47.6	55.0	58.0	58.0	1036-1037 bp	1.0	72.6
trnK-UUU-rbcL_p4	AGCCGAGTACTCTACCATTGA	TGCTTGCGAAGTATGGAAGG	47.6	50.0	58.0	58.3	1004-1005 bp	1.0	70.8
trnK-UUU-rbcL_p5	AGCCGAGTACTCTACCATTGA	GGCTAGCAAATGGAGTCCTG	47.6	55.0	58.0	58.3	1034-1035 bp	1.0	70.3