Hotspot

ndhF-rpl32

SSC · 116410-117261 · 852 bp

Back to Ammannia

Pi 0.0347

MarkerSeek score 71.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 852
variable_sites: 42
pi: 0.0347
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TAGGTCGCGTGAACCAAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	1909-2017 bp	1.0	83.5
ndhF-rpl32_p2	TCGCGTGAACCAAAAACCTA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	1905-2013 bp	1.0	83.5
ndhF-rpl32_p3	TCGCGTGAACCAAAAACCTA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	1906-2014 bp	1.0	83.5
ndhF-rpl32_p4	GAAGAGGGAATTGTGCCGAT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	2140-2248 bp	1.0	83.2
ndhF-rpl32_p5	GAAGAGGGAATTGTGCCGAT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	2141-2249 bp	1.0	83.2