Hotspot

accD-psaI

LSC · 62200-62858 · 659 bp

Back to Ammannia

Pi 0.0269

MarkerSeek score 70.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 659
variable_sites: 26
pi: 0.0269
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGATCCAATCGTACCACGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.0	58.0	831-857 bp	1.0	74.7
accD-psaI_p2	TTGATCCAATCGTACCACGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.0	57.9	837-863 bp	1.0	74.0
accD-psaI_p3	TTTGATCCAATCGTACCACGT	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.7	58.0	832-858 bp	1.0	72.4
accD-psaI_p4	TTTGATCCAATCGTACCACGT	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.7	57.9	838-864 bp	1.0	71.8
accD-psaI_p5	TCCAATCGTACCACGTAATCC	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.6	58.0	827-853 bp	1.0	71.5