Hotspot

trnT-GGU-psbD

LSC · 33974-35414 · 1441 bp

Back to Ammannia

Pi 0.0286
MarkerSeek score 68.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1441
variable_sites
60
pi
0.0286
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GGCGTAAGTCATCGGTTCAA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.3 53.0 1497-1517 bp 1.0 45.9
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG TTTACCAAGGGCTATAGTCA 55.0 40.0 58.1 53.0 1520-1540 bp 1.0 45.8
trnT-GGU-psbD_p3 TCAGTGGTAGAGTAACGCCA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1523-1543 bp 1.0 45.8
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG TTACCAAGGGCTATAGTCAT 55.0 40.0 58.1 52.8 1519-1539 bp 1.0 45.8
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA TTACCAAGGGCTATAGTCAT 50.0 40.0 58.1 52.8 1522-1542 bp 1.0 45.8