Hotspot

accD

Genome · 57839-60565 · 2727 bp

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Pi 0.00453

MarkerSeek score 48.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 2727
variable_sites: 29
pi: 0.00453
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCGATTCACGAGGTTCTGAT	GGATCAAGACTTGTTTTTGGGG	45.0	45.5	56.4	57.7	2883-2979 bp	0.833333	44.5
accD_p2	TGTGCAAAGGATATCACTCGA	GGATCAAGACTTGTTTTTGGGG	42.9	45.5	57.1	57.7	2900-2996 bp	0.833333	42.3
accD_p3	CGAGTTCTTCAAGTCGTTCCA	GGATCAAGACTTGTTTTTGGGG	47.6	45.5	58.3	57.7	2948-2996 bp	0.5	33.6
accD_p4	CGAGTTCTTCAAGTCGTTCC	GGATCAAGACTTGTTTTTGGGG	50.0	45.5	56.5	57.7	2948-2996 bp	0.5	31.8
accD_p5	CGAGTTCTTCAAGTCGTTCCA	TCAAGACTTGTTTTTGGGGA	47.6	40.0	58.3	55.0	2945-2993 bp	0.5	27.5