Hotspot

chlL-trnH-GUG

Genome · 135414-136090 · 677 bp

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Pi 0.00473

MarkerSeek score 62.30

Primer available yes

Label chlL-trnH-GUG

Feature Metadata

label_name: chlL-trnH-GUG
feature_type: igs
region: Genome
length_bp: 677
variable_sites: 7
pi: 0.00473
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL-trnH-GUG_p1	ACCAATTCCGCCTTTTCCAT	ATTATTTGGGGCGGACGTAG	45.0	50.0	58.1	57.8	798-814 bp	0.333333	51.0
chlL-trnH-GUG_p2	ACCAATTCCGCCTTTTCCAT	CTATTATTTGGGGCGGACGT	45.0	50.0	58.1	57.8	800-816 bp	0.333333	51.0
chlL-trnH-GUG_p3	GTCTGGCTAGGGCAATTGAA	ATTATTTGGGGCGGACGTAG	50.0	50.0	57.9	57.8	841-857 bp	0.333333	50.5
chlL-trnH-GUG_p4	GTCTGGCTAGGGCAATTGAA	CTATTATTTGGGGCGGACGT	50.0	50.0	57.9	57.8	843-859 bp	0.333333	50.5
chlL-trnH-GUG_p5	TTTTCACCACGTCTGGCTAG	ATTATTTGGGGCGGACGTAG	50.0	50.0	57.8	57.8	851-867 bp	0.333333	50.2