Hotspot

trnR-ACG-trnN-GUU

Genome · 110071-110544 · 474 bp

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Pi 0.00464

MarkerSeek score 44.90

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: Genome
length_bp: 474
variable_sites: 6
pi: 0.00464
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	57.8	57.7	609 bp	1.0	76.6
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	CCGATTGGTCGTAGGTTCAA	55.0	50.0	57.8	57.6	571 bp	1.0	76.1
trnR-ACG-trnN-GUU_p3	TTTTTGGAGGCACTTCTGGG	TTCCTCAGTAGCTCAGTGGT	50.0	50.0	58.3	57.7	665 bp	1.0	75.9
trnR-ACG-trnN-GUU_p4	TTTTTGGAGGCACTTCTGGG	CCGATTGGTCGTAGGTTCAA	50.0	50.0	58.3	57.6	627 bp	1.0	75.4
trnR-ACG-trnN-GUU_p5	GAACAGATTCCGTGGAGACG	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	58.4	57.7	641 bp	1.0	75.3