Hotspot

trnV-GAC-rrn16

Genome · 100934-102313 · 1380 bp

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Pi 0.0146

MarkerSeek score 59.60

Primer available yes

Label trnV-GAC-rrn16

Feature Metadata

label_name: trnV-GAC-rrn16
feature_type: igs
region: Genome
length_bp: 1380
variable_sites: 40
pi: 0.0146
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rrn16_p1	ACCTTGACGTGGTGAAAGTC	CCGACTTGCATGTGTTAAGC	50.0	50.0	58.1	57.8	1107-1491 bp	1.0	84.0
trnV-GAC-rrn16_p2	CCTTGACGTGGTGAAAGTCA	CCGACTTGCATGTGTTAAGC	50.0	50.0	58.1	57.8	1106-1490 bp	1.0	84.0
trnV-GAC-rrn16_p3	TGACGTGGTGAAAGTCATCG	CCGACTTGCATGTGTTAAGC	50.0	50.0	58.2	57.8	1103-1487 bp	1.0	82.7
trnV-GAC-rrn16_p4	ACCTTGACGTGGTGAAAGTC	CGTCCGACTTGCATGTGTTA	50.0	50.0	58.1	58.6	1110-1494 bp	1.0	81.4
trnV-GAC-rrn16_p5	CCTTGACGTGGTGAAAGTCA	CGTCCGACTTGCATGTGTTA	50.0	50.0	58.1	58.6	1109-1493 bp	1.0	81.4