Hotspot

psbM-trnD-GUC

Genome · 28465-29805 · 1341 bp

Back to Amentotaxus

Pi 0.00303

MarkerSeek score 59.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: Genome
length_bp: 1341
variable_sites: 10
pi: 0.00303
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CAATTGCTTTGACTCACCGT	TTAGAGTACCGCCCTGTCAA	45.0	50.0	56.9	58.1	1492-1502 bp	1.0	72.0
psbM-trnD-GUC_p2	CAATTGCTTTGACTCACCGT	ATTGGTTAGAGTACCGCCCT	45.0	50.0	56.9	58.1	1497-1507 bp	1.0	71.5
psbM-trnD-GUC_p3	TCAATTGCTTTGACTCACCG	TTAGAGTACCGCCCTGTCAA	45.0	50.0	56.7	58.1	1493-1503 bp	1.0	69.7
psbM-trnD-GUC_p4	CAATTGCTTTGACTCACCGTT	TTAGAGTACCGCCCTGTCAA	42.9	50.0	57.6	58.1	1492-1502 bp	1.0	69.7
psbM-trnD-GUC_p5	TCAATTGCTTTGACTCACCG	ATTGGTTAGAGTACCGCCCT	45.0	50.0	56.7	58.1	1498-1508 bp	1.0	69.3