Hotspot

petN-psbM

Genome · 27486-28359 · 874 bp

Back to Amentotaxus

Pi 0.00328

MarkerSeek score 56.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: Genome
length_bp: 874
variable_sites: 8
pi: 0.00328
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGCTGCTCTAATGGTCGTTT	ACGGTGAGTCAAAGCAATTG	50.0	45.0	58.3	56.9	953-963 bp	1.0	70.9
petN-psbM_p2	CTTGGGCTGCTCTAATGGTC	ACGGTGAGTCAAAGCAATTG	55.0	45.0	58.3	56.9	957-967 bp	1.0	70.4
petN-psbM_p3	GGCTGCTCTAATGGTCGTTT	CGGTGAGTCAAAGCAATTGA	50.0	45.0	58.3	56.7	952-962 bp	1.0	68.6
petN-psbM_p4	GGCTGCTCTAATGGTCGTTT	AACGGTGAGTCAAAGCAATTG	50.0	42.9	58.3	57.6	954-964 bp	1.0	68.6
petN-psbM_p5	CTTGGGCTGCTCTAATGGTC	CGGTGAGTCAAAGCAATTGA	55.0	45.0	58.3	56.7	956-966 bp	1.0	68.1